Journal: Cell Discovery
Article Title: Single-cell RNA sequencing identifies an Il1rn + /Trem1 + macrophage subpopulation as a cellular target for mitigating the progression of thoracic aortic aneurysm and dissection
doi: 10.1038/s41421-021-00362-2
Figure Lengend Snippet: a Trem1 was highly expressed in the macrophage subpopulation. b Schematic illustration of the treatment procedure. Two biological replicates ( n = 8 for each replicate) were performed for each group. All measurements were performed after 28 days of treatment. c Survival curves of mice treated with mLR12 or vehicle (saline solution). d Summary of the normal, TAAD, and rupture rates in each group. e mLR12 treatment could significantly decrease the aortic rupture rate. f Quantification of plasma levels of sTREM1. n = 6 for each group. g Quantification of the mRNA expression of Tnfa , Il1b , Il6 , Trem1 , and Cd11b in the aorta by qPCR ( n = 3). h Immunofluorescence staining for Cd11b + Trem1 + cells in mouse aorta of the mLR12 treatment group. i Immunofluorescence staining for Cd11b + Trem1 + cells in mouse aorta of the BAPN + vehicle group. j The percentage of the Cd11b + Trem1 + cells in each sample was calculated based on the immunofluorescence staining. Wilcoxon rank-sum test, P = 0.0004. n = 9 for each group. k Immunofluorescence staining for CD11b + TREM1 + cells in aortic adventitia of human TAAD patients. l Immunofluorescence staining for CD11b + TREM1 + cells in aortic adventitia of healthy donors. m The expression of TREM1 in aortic tissues of human TAAD patients ( n = 8) and healthy donors ( n = 6) measured by bulk RNA-seq. n The expression of TREM1 in CD11b + and CD11b − cells sorted from the aortic tissues of TAAD patients ( n = 4) measured by bulk RNA-seq. In f and g , all values are means ± SEM. * P < 0.05, ** P < 0.01, n.s. not significant, Kruskal–Wallis test followed by multiple pairwise comparisons between groups.
Article Snippet: The secreted soluble form of Trem1 (sTrem1) was measured using Mouse/Rat TREM1 Quantikine ELISA Kit (R&D Systems, Cat. No. MTRM10) according to the manufacturer’s instructions.
Techniques: Saline, Clinical Proteomics, Expressing, Immunofluorescence, Staining, RNA Sequencing
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